Sterile Barrier Systems Testing Company: Bacterial endotoxins are also known as "pyrogens". It is a unique structure on the cell wall of Gram-negative bacilli. Bacteria will not release it when they are alive, and will only be released outside the cell when they die. Express a variety of toxic effects, in order to distinguish from exotoxins, called endotoxins. Bacterial endotoxin is a high-content complex composed of lipopolysaccharide (LPS), protein and phospholipids. The toxic component is mainly lipid A (Lipid A), and maintaining A is an indispensable part of LPS activity, which can be regarded as the "Bioactive Center". Located in the outer layer of the cell wall, the cell wall is covered with viscous. The endotoxins of various bacteria are weakly toxic and roughly the same, which can cause fever, microcirculation disturbance, endotoxin shock, disseminated intravascular coagulation, etc. Endotoxin is heat-resistant, stable, and has weak antigenicity. The body can produce antibodies, but there is no neutralizing effect, and anti-toxins can be formed. After formaldehyde treatment, they cannot become toxic.

　　1. Sterile barrier system testing company: What is the detection method of bacterial endotoxin?

　　Sterile barrier system testing company: In the 1960s, ancient marine organisms called "marine living fossils" were discovered, and their blood would undergo a specific coagulation reaction after contact with endotoxin. After a lot of research and experiments, from its blood The "reagent" for detecting endotoxin is extracted from the The unit of endotoxin detection is EU/ml.

　　Sterile barrier system testing company: At present, the detection methods of bacterial endotoxin are as follows:

　　For the gel method, the samples and reagents are mixed in a test tube, using a dry bath, and do not shake during incubation at 37 1 to 60 2 min. Immediately after the heating is completed, the test tube is slowly tilted 180 degrees. Once the gel is formed, if it maintains its integrity without deforming or shrinking, the result will be confirmed as positive; if the gel does not form, it will be confirmed as negative. During the test, multiple dilutions (usually 2-fold) are performed on a series of samples to confirm whether the result of each sample is positive. The maximum effective dilution factor or the minimum concentration determined to be positive is called the end point.

　　Sterile Barrier Systems Testing Company: Bacterial Endotoxin Detection Lipopolysaccharide (LPS) biomarker assay can detect the type of LPS in the sample, sensitively detect the concentration of LPS in the sample, but cannot accurately detect the concentration of endotoxin that plays a biological role .

　　Sterile barrier system testing company: Enzyme-linked immunoassay (ELISA) and ELISA are currently emerging endotoxin detection methods. Antigen-antibody-based enzyme-linked immunoassays are produced by heating protoplasmic macrophages. Source substances (such as tumor necrosis (TNF) factor interleukin 1 (IL-1), etc. were determined.

　　Sterile barrier system testing company: Biosensor detection method and biosensor are also emerging bacterial endotoxin detection methods, most of which are based on the LPS coagulation reaction of amoeba lysate and endotoxin in the catfish test method. This bacterial endotoxin detection method has the characteristics of fast measurement speed, high sensitivity, and low detection coefficient. At present, there are many researches on the detection of endotoxin with biosensors at home and abroad.