How to ensure the accuracy of microbial test results in bioburden testing? How to do?

In the process of bioburden detection and microbial inspection, it is necessary to ensure the cleanliness of the testing environment (such as ultra-clean platform, testing room) and the instruments used, and at the same time ensure the standardization of personnel's operations, try to ensure its sterility, and prevent If the results are wrong due to human error, it should be cultivated at a suitable temperature to provide a basis for reasonable decision-making and production guidance.

In order to ensure the accuracy of the test results, we should do the following.

1. Every time you prepare, pay attention to whether the production date is within the shelf life, check the date of opening the bottle and the seal of the bottle mouth to ensure that there is no deterioration or moisture absorption.

2. When preparing the culture medium, the weighing should be accurate, including the packaging of saline.

3. Make sure that the prepared medium is sterilized now, and the unsterilized prepared medium cannot be stored for a long time, let alone overnight.

4. When sterilizing, it is necessary to ensure constant temperature and constant pressure, and at the same time, it is necessary to ensure an effective sterilization time.

5. The sterilized culture medium should be stored in the refrigerator for one week, generally no more than 3 days. And to follow the "first in, first out" principle, the first to prepare first.

6. When using, according to the use of the shift, first melt the medium into a liquid state with a water bath, and then lower the temperature of the water bath in time (the melted one can be taken out of the water bath and covered with a water bath cover) for later use. Never leave the culture medium at high temperature for an extended period of time.

7. When doing microbiological testing of products, pour the medium at about 45°C, do not overheat, so as not to scald the bacteria to death; not too cold, the melted medium will clump and solidify, making it inconvenient to observe the results.

8. Each bottle of medium should be blank.

9. Try to concentrate on sample preparation, avoid frequently opening the cork of the same bottle of medium, which will increase the chance of medium contamination and cause errors.

10. If there is too little medium left, pour into a blank plate first.

Maintenance of Bioburden Testing Environment:

(1) During the test, the windows and air conditioner should be closed, or the room should be disinfected with alcohol spray to avoid the impact of indoor air circulation on the environment in the ultra-clean platform (it is better to operate in a constant temperature and humidity sterile room with a ventilation system ).

(2) If the inspection is carried out in the raw material microbiology inspection room, the ultraviolet light should be turned on regularly to disinfect the room.

Small environment for bioburden detection (clean bench):

1. Regularly clean the primary filter and replace it in time.

2. Before the test, clean the inside of the ultra-clean bench, wipe it with 75% alcohol, and turn on the ultraviolet light of the ultra-clean bench half an hour in advance to disinfect the internal environment of the ultra-clean bench.

3. Turn off the UV lamp, turn on the fan, and adjust the appropriate air intake. The air volume should not be too low.

4. After each test, the inside of the ultra-clean platform must be cleaned and disinfected with 75% alcohol. 5. The UV lamp should be replaced in time according to its service life.

6. At the same time as the detection, an environmental blank for the corresponding colony detection should be made.

7. Selection of detection area:

Generally, aseptic operation is performed in the area 1/3-2/3 away from the outer edge of the ultra-clean platform;

Operate in the outer flame protection area of the alcohol lamp flame.

Cleanliness of Bioburden Testing Instruments:

1. Glassware: Sterilized by dry heat sterilization.

2. Scissors, spoons and other items. Instruments used for testing should be used exclusively for testing and should not be mixed with other operating instruments. When in use, first sterilize with 75% alcohol, and then sterilize with fire.

3. The inoculation needle (ring) is sterilized by burning, but it should be noted that the inoculation needle (ring) should be cooled before testing.

Collection and testing of samples for bioburden testing:

1. Ensure the sterility of the sampling instrument.

(1) Glassware: Use dry heat to sterilize to ensure a constant temperature and sufficient time (but not too long, which may easily cause glassware to break and be scrapped).

(2) Sampling basket: spray with 75% alcohol before use.

(3) Sampling spoon: To ensure its cleaning and disinfection, wipe it with 75% alcohol after cleaning.

(4) Sampling bag: firstly, it can be wiped and disinfected with alcohol, and then sterilized by ultraviolet light in the ultra-clean bench (the packaging workshop should be sterilized by ultraviolet light in the transmission window).

(5) The surface of the electronic scale is disinfected with 75% alcohol.