Medium detection method:

For a long time, the road of domestic medium testing has been bumpy. As some domestic media suppliers have invested more and more energy in the R&D and production of media, many excellent products have gradually surpassed imported brands and become the mainstream, which also allows us to see the hope of domestic brands.

Media detection method:

Clarity inspection method for medium detection: Clarity is to check the turbidity of the drug solution. If there are fine particles in the solution, when direct light passes through the solution, light scattering and light absorption will occur, resulting in a slightly turbid solution, so the clarity can reflect the quality and production process level of the drug to a certain extent.

The nutrients in the medium test can only be absorbed by the cells and the cells can grow and proliferate only if they are completely soluble in water. Therefore, whether the culture medium is dissolved and whether the culture medium is transparent directly affects the use of the culture medium detection. The solubility of the cell culture medium can be judged by checking the clarity of the medium dissolved in water. In this method, a turbidity standard solution with a prescribed number of grades is compared with a sample solution to determine the clarity or turbidity of the cell culture fluid.

Determination of the pH value of the medium test: the normal functions of various cells and all biochemical reactions are carried out in a suitable and stable acid-base environment, so the pH value of the cell culture medium is an important parameter for cell growth, pH Low or high values will affect cell growth.

In industrial use, since the traditional cell culture medium test contains pH indicators such as phenol red, the pH value is usually determined by observing the color of the culture solution with "eyes", or simply using pH test paper to determine the pH value. However, the above-mentioned method has relatively large subjective factors, which affect the correct determination, and in the process of preparation, there may be certain differences in water quality in different regions. Using pH meter culture medium to test can eliminate human subjective judgment, and the accuracy is higher. In addition, during the detection process, the exposure time of the medium added with sodium bicarbonate in the air will also affect the accuracy of the measured pH value.