Cell Medium Assay Method

1 Clarity of culture medium test

Water is a solvent for cell culture media. In the analysis of nutrient components in the cell culture medium test, only one that can be completely dissolved in water can be absorbed by the cells, and the development of cells can promote growth and proliferation. Therefore, whether the cell culture medium test can dissolve and whether the culture medium is transparent and clear will directly affect Media used. This project is to judge the clarity of these cell culture media by checking the clarity of the cell culture media dissolved in water.

2 Medium test pH value

Mammalian cells need a suitable pH for growth and development. If the pH is too high or not too low, it will affect the death of these cells. The determination of the pH value can also be based on the inspection to find the batch-to-batch difference of the cell culture medium. Tsingda Tianyi standard management stipulates to take a specified amount of the test product, dissolve it with water for injection (water temperature 20°C-30°C) to 1L, add a specified amount of sodium bicarbonate to the above analysis solution, and use a solution with a pH value similar to that of the cell culture medium. Two important standard buffer solutions calibrated pH meter for research determination. According to the National Pharmacopoeia of the People's Democratic Republic of China in 2005, Appendix VI H of Part Two; add a specified amount of sodium bicarbonate to the above working solution.

3. The reduction of the dry mass fraction of the culture medium

The cell culture medium is hygroscopic, and the moisture content in the air will rise quickly, and the dry weight of the mass fraction of the product represents the moisture content. Cell culture medium is a microbial preparation whose rich nutrients are conducive to the growth of microorganisms. Controlling the moisture in the cell culture medium can prevent the reproduction of microorganisms and maintain the nutrient content in the cell culture medium.

4.Medium Test Osmotic Pressure

The phenomenon that a solvent diffuses from a low-concentration solution to a high-concentration solution through a semipermeable membrane is called osmosis, and the pressure required to prevent osmosis is called osmotic pressure. Cells should live in an isotonic environment, and animal cells maintain osmotic balance with the help of K+ and Na+. Most cells have some tolerance to osmotic pressure. However, the high osmotic pressure of the medium can easily cause the cells to dehydrate to suo, while the low osmotic pressure of the medium can easily cause the cells to swell and rupture, so the osmotic pressure range of the medium should be controlled.